Biological Sciences & Biotechnologyhttp://irgu.unigoa.ac.in/drs/handle/unigoa/32024-03-29T06:50:27Z2024-03-29T06:50:27ZEvaluating the carnivorous efficacy of Utricularia aurea (Lamiales: Lentibulariaceae) on the larval stages of Anopheles stephensi, Culex quinquefasciatus, and Aedes aegypti (Diptera: Culicidae)Mohanty, A.K.Govekar, A.de Souza1, C.Mohapatra, A.Janarthanam, M.K.Vukanti, R.Montemarano, J.Nina, B.B.http://irgu.unigoa.ac.in/drs/handle/unigoa/72782024-03-27T09:02:07Z2024-01-01T00:00:00ZEvaluating the carnivorous efficacy of Utricularia aurea (Lamiales: Lentibulariaceae) on the larval stages of Anopheles stephensi, Culex quinquefasciatus, and Aedes aegypti (Diptera: Culicidae)
Mohanty, A.K.; Govekar, A.; de Souza1, C.; Mohapatra, A.; Janarthanam, M.K.; Vukanti, R.; Montemarano, J.; Nina, B.B.
The emergence of insecticide resistance in mosquitoes necessitates the exploration and validation of sustainable biological strategies for controlling mosquitoes in their natural habitats. We assessed the predatory effect of Utricularia aurea Lour (Lamiales: Lentibulariaceae), an aquatic carnivorous plant found in the Indian subcontinent, Japan, and Australia, on 4 instars of Anopheles stephensi Liston, Culex quinquefasciatus Say, and Aedes aegypti Linn (Diptera: Culicidae), in the laboratory and field settings. In the laboratory setting, predation of larvae by U. aurea was highest during the first hour when it predated 45 percent, 61 percent, and 58 percent of first instars of An. stephensi, Cx. quinquefasciatus, and, Ae. aegypti, respectively, and, within 12 h, U. aurea preyed upon approx. 95 percent of the first, second, and third instars of the 3 mosquito species, approx. 80 percent of the fourth instars of An. stephensi and Ae. aegypti, and approx. 60 percent of fourth instars of Cx. quinquefasciatus. The predatory effect of U. aurea varied with mosquito species and instar. Broadly, predation risk declined with the increase of the instar size. In the field setting, at the end of 16 days, U. aurea predated 76 percent and 71 percent of the immature An. stephensi and Ae. aegypti, respectively. Our findings suggest U. aurea can be utilized as a potential biocontrol agent for controlling mosquito larvae in natural habitats; however, the current claim warrants additional investigations in a variety of natural habitats.
2024-01-01T00:00:00ZBacterial diversity and community structure of salt pans from Goa, IndiaGawas, P.Kerkar, S.http://irgu.unigoa.ac.in/drs/handle/unigoa/72682024-03-12T06:13:21Z2023-01-01T00:00:00ZBacterial diversity and community structure of salt pans from Goa, India
Gawas, P.; Kerkar, S.
In Goa, salt production from the local salt pans is an age-old practice. These salt pans harbor a rich diversity of halophilic microbes with immense biotechnological applications, as they tolerate extremely harsh conditions. Detecting the existence of these microbes by a metabarcoding approach could be a primary step to harness their potential. Three salt pans viz. Agarwado, Curca, and Nerul adjoining prominent estuaries of Goa were selected based on their unique geographical locations. The sediments of these salt pans were examined for their bacterial community and function by 16S rRNA amplicon-sequencing. These salt pans were hypersaline (400-450 PSU) and alkaline (pH 7.6-8.25), with 0.036-0.081 mg/L nitrite, 0.0031-0.016 mg/L nitrate, 6.66-15.81 mg/L sulfate, and 20.8-25.6 mg/L sulfide. The relative abundance revealed that the Pseudomonadota was dominant in salt pans of Nerul (13.9 percent), Curca (19.6 percent), and Agarwado (32.4 percent). The predominant genera in Nerul, Curca, and Agarwado salt pan sediments were Rhodopirellula (1.12 percent), Sulfurivermis (1.28 percent), and Psychrobacter (25.5 percent) respectively. The highest alpha diversity (Shannon-diversity Index) was observed in the Nerul salt pan (4.8) followed by Curca (4.3) and Agarwado (2.03). Beta diversity indicated the highest dissimilarity between Agarwado and the other two salt pans (0.73) viz. Nerul and Curca and the lowest dissimilarity was observed between Nerul and Curca salt pans (0.48). Additionally, in the Agarwado salt pan, 125 unique genera were detected, while in Nerul 119, and in Curca 28 distinct genera were noted. The presence of these exclusive microorganisms in a specific salt pan and its absence in the others indicate that the adjacent estuaries play a critical role in determining salt pan bacterial diversity. Further, the functional prediction of bacterial communities indicated the predominance of stress adaptation genes involved in osmotic balance, membrane modification, and DNA repair mechanisms. This is the first study to report the bacterial community structure and its functional genes in these three salt pans using Next-Generation Sequencing. The data generated could be used as a reference by other researchers across the world for bioprospecting these organisms for novel compounds having biotechnological and biomedical potential.
2023-01-01T00:00:00ZSuperwettable surfaces and factors impacting microbial adherence in microbiologically-influenced corrosion: A reviewRane, D.Kerkar, S.Roy, S.R.Kowshik, M.http://irgu.unigoa.ac.in/drs/handle/unigoa/72692024-03-12T06:14:09Z2024-01-01T00:00:00ZSuperwettable surfaces and factors impacting microbial adherence in microbiologically-influenced corrosion: A review
Rane, D.; Kerkar, S.; Roy, S.R.; Kowshik, M.
Microbiologically-influenced corrosion (MIC) is a common operational hazard to many industrial processes. The focus of this review lies on microbial corrosion in the maritime industry. Microbial metal attachment and colonization are the critical steps in MIC initiation. We have outlined the crucial factors influencing corrosion caused by microorganism sulfate-reducing bacteria (SRB), where its adherence on the metal surface leads to Direct Electron Transfer (DET)-MIC. This review thus aims to summarize the recent progress and the lacunae in mitigation of MIC. We further highlight the susceptibility of stainless steel grades to SRB pitting corrosion and have included recent developments in understanding the quorum sensing mechanisms in SRB, which governs the proliferation process of the microbial community. There is a paucity of literature on the utilization of anti-quorum sensing molecules against SRB, indicating that the area of study is in its nascent stage of development. Furthermore, microbial adherence to metal is significantly impacted by surface chemistry and topography. Thus, we have reviewed the application of super wettable surfaces such as superhydrophobic, superhydrophilic, and slippery liquid-infused porous surfaces as "anti-corrosion coatings" in preventing adhesion of SRB, providing a potential avenue for the development of practical and feasible solutions in the prevention of MIC. The emerging field of super wettable surfaces holds significant potential for advancing efficient and practical MIC prevention techniques.
2024-01-01T00:00:00ZIsolation and Characterization of Cationic Peptides from Selected PlantsSreekala, S.http://irgu.unigoa.ac.in/drs/handle/unigoa/72652024-03-09T07:10:47Z2022-01-01T00:00:00ZIsolation and Characterization of Cationic Peptides from Selected Plants
Sreekala, S.
2022-01-01T00:00:00Z