http://irgu.unigoa.ac.in/drs/handle/unigoa/20 Wed, 13 May 2026 12:49:20 GMT 2026-05-13T12:49:20Z http://irgu.unigoa.ac.in/drs/handle/unigoa/7818 Metagenomic studies reveal diverse microbial community in the developmental stages of highly adaptable malarial vector Anopheles stephensi liston Pereira, M.H.; Tyagi, S.; Mohanty, A.; Garg, S.; Ashwani Kumar Anopheles stephensi, a highly adaptable malaria vector species, continues to expand its range from South Asia to Sub-Saharan Africa, posing a serious global public health concern. In India, it serves as the principal urban vector of both Plasmodium falciparum and P. vivax. Conventional control measures reliant on chemical insecticides have raised issues of resistance, highlighting the need for alternative strategies such as microbiota-mediated vector control. This study aimed to test the hypothesis that a subset of bacterial taxa persist across developmental stages of An. stephensi, representing potential candidates for transstadial transmission and future paratransgenic manipulation. Using both culture-based data and next-generation sequencing (NGS) approaches targeting the 16 S rRNA gene (V3-V4 region), we characterized bacterial communities from breeding water, larvae, pupae, and adult mosquitoes (male and female) collected in Goa, India. Across all developmental stages, Proteobacteria and Firmicutes were the dominant phyla, while 15 bacterial genera formed the putative core microbiome shared by greater than or equal to 80 percent of stages at greater than or equal to 0.1 percent abundance. Among these, Pseudomonas (adult males: 11.5 percent, pupae: 3.2 percent), Exiguobacterium, Acinetobacter, Psychrobacter, and Asticcacaulis were consistently detected, together contributing approximately 30 percent of total microbial composition. Alpha diversity indices indicated higher richness and evenness in pupae and adults than in larvae, suggesting microbial enrichment during metamorphosis. Beta diversity and PCoA analyses clustered pupal and adult stages distinctly from larvae and breeding water, confirming selective microbial retention through development. These findings reveal that An. stephensi harbors a stable, stage-spanning core microbiome dominated by metabolically versatile genera with potential for transstadial persistence. The dominance of Pseudomonas across life stages supports its candidacy for paratransgenic applications aimed at disrupting malaria transmission. This work provides the first integrated culture-NGS baseline of An. stephensi microbiota from India, offering essential insight for microbiome-based vector control strategies. Thu, 01 Jan 2026 00:00:00 GMT http://irgu.unigoa.ac.in/drs/handle/unigoa/7818 2026-01-01T00:00:00Z http://irgu.unigoa.ac.in/drs/handle/unigoa/7801 Efficacy of nonantimicrobial drugs against Enterococcus faecalis using confocal laser scanning microscopy - An in vitro study Ferrao, T.M.; Ataide, I.; Lambor, R.; Kashyap, S.; Biswas, S.; Salkar, K.S.; Charya, L.S. Enterococcus faecalis is a key contributor to persistent endodontic infections and often shows resistance to conventional medicaments such as calcium hydroxide. Growing antimicrobial resistance has led to interest in repurposing nonantibiotic drugs such as nonsteroidal anti-inflammatory drugs and proton-pump inhibitors that have shown promising antimicrobial effects against E. faecalis. The study aimed to evaluate the in vitro antibiofilm activity of different combinations of nonantimicrobial drugs with and without calcium hydroxide against E. faecalis. Thu, 01 Jan 2026 00:00:00 GMT http://irgu.unigoa.ac.in/drs/handle/unigoa/7801 2026-01-01T00:00:00Z http://irgu.unigoa.ac.in/drs/handle/unigoa/7796 Field evaluation of bacterial wilt resistant lines and identification of promising bacterial wilt resistant varieties for Coastal region Ramesh, R.; D'Souza, M.; Asolkar, T.; Achari, G.; Gaitonde, S.; Thangam, M. Cultivation of brinjal in Coastal regions of India is severely affected by bacterial wilt disease caused by Ralstonia solanacearum. In this study, recombinant inbred lines were developed and screened for bacterial wilt resistance. Seventeen promising resistant lines were evaluated in the field for two years and the results indicated these lines were resistant to bacterial wilt. Lines 262-4, 5-12-1, 66-8-1, 205-1, 12-2-4 and 28-8-5 were promising among the purple types (0 to 4 percent wilt and yield 0.51 to 0.93 kg/ plant). Among the green types, lines 5-8-1, 93-8-1 and 92-3-7 were promising (0 to 5 percent wilt and yield 0.64 to 0.79 kg/ plant). Demonstration of eight lines in farmer's field indicated that lines 262-4, 5-12-1, 205-1, 5-8-1 and 12-2-4 were least infected (3 to 17 percent). Based on these studies, four purple lines 262-4, 5-12-1, 66-8-1 and 205-1 were released as bacterial wilt resistant brinjal varieties in the State of Goa. Fri, 01 Jan 2021 00:00:00 GMT http://irgu.unigoa.ac.in/drs/handle/unigoa/7796 2021-01-01T00:00:00Z http://irgu.unigoa.ac.in/drs/handle/unigoa/7797 Evaluation of bacterial wilt resistant brinjal lines and field performance of bacterial wilt resistant brinjal varieties in the coastal region Ramesh, R.; D'Souza, M.; Asolkar, T.; Achari, G.; Maruthadurai, R. Bacterial wilt disease in brinjal caused by Ralstonia solanacearum is a major production constraint in Coastal regions of India. Among various management strategies, cultivation of resistant varieties is the most effective one. In this study, we screened 17 recombinant inbred lines for bacterial wilt resistance in green house followed by evaluation in the field for two years. Fifteen lines showed resistance to bacterial wilt. Two lines, 27-7-2 and 42-7-1 designated as Goa Brinjal-5 and Goa Brinjal-6 were released for bacterial wilt management as they exhibited 0 to 0.83 percent wilt and a yield of 0.76-0.84 Kg/plant. Field evaluation of six bacterial wilt resistant varieties for 2 years indicated that the wilt incidence was less than 3 percent while it was above 95 percent in the susceptible cultivars. Demonstration of six varieties in farmer's field indicated that wilt incidence was less than 4 percent in the varieties compared to 77-82 percent in susceptible cultivars. Sun, 01 Jan 2023 00:00:00 GMT http://irgu.unigoa.ac.in/drs/handle/unigoa/7797 2023-01-01T00:00:00Z