Development of primary cell culture from Scylla serrata

Sashikumar, A.; Desai, P.V.

dc.contributor.author	Sashikumar, A.
dc.contributor.author	Desai, P.V.
dc.date.accessioned	2015-06-03T10:01:31Z
dc.date.available	2015-06-03T10:01:31Z
dc.date.issued	2008
dc.identifier.citation	Cytotechnology. 56(3); 2008; 161-169.	en_US
dc.identifier.uri	http://dx.doi.org/10.1007/s10616-008-9152-1
dc.identifier.uri	http://irgu.unigoa.ac.in/drs/handle/unigoa/2217
dc.description.abstract	This paper reports for the first time, the Primary cell culture of hepatopancreas from edible crab Scylla serrata using crab saline, L-15 (Leibovitz), 1 x L-15 + crab saline, 2 x L-15 + crab saline, 3 x L-15 and citrate buffer without any serum. We could isolate and maintain E (Embryonalzellen), F (Fibrenzellen), B (Blasenzellen), R (Restzellen) and G (Granular cells). Upon seeding the hepatopancreatic E, F, B, and R cells showed different survival pattern over time than granular cells. A modified L-15 (3x) medium supported the best survival of hepatopancreatic E, F B, and R cells in in-vitro culture. However granular cells could be maintained for 184 days with L-15 (1x) + crab saline. Fetal bovine serum was not effective additive and hampered cell viability in present study.	en_US
dc.publisher	Springer Verlag (Germany)	en_US
dc.subject	Zoology	en_US
dc.title	Development of primary cell culture from Scylla serrata	en_US
dc.type	Journal article	en_US
dc.identifier.impf	y