Isolation and characterization of Listeria species from raw and processed meats

Doijad, S.P.; Vaidya, V.; Garg, S.; Kalekar, S.; Rodrigues, J.; D'Costa, D.; Bhosle, S.; Barbuddhe, S.B.

dc.contributor.author	Doijad, S.P.
dc.contributor.author	Vaidya, V.
dc.contributor.author	Garg, S.
dc.contributor.author	Kalekar, S.
dc.contributor.author	Rodrigues, J.
dc.contributor.author	D'Costa, D.
dc.contributor.author	Bhosle, S.
dc.contributor.author	Barbuddhe, S.B.
dc.date.accessioned	2015-06-04T02:52:36Z
dc.date.available	2015-06-04T02:52:36Z
dc.date.issued	2010
dc.identifier.citation	Journal of Veterinary Public Health Sciences. 8(2); 2010; 83-88.	en_US
dc.identifier.uri	http://www.iavphs.org/jvph/index.php/none/article/view/17
dc.identifier.uri	http://irgu.unigoa.ac.in/drs/handle/unigoa/2484
dc.description.abstract	A total of 109 meat and meat product samples (50 beef, 52 pork sausages and 7 pork) were examined for the presence of Listeria species. Isolates were confirmed by biochemical characterization and haemolysis on blood agar. A total of 25 (22.94 percent) isolates were recovered. Of these, 7 (6.42 percent) were L. monocytogenes, while one (0.9 percent) was L. ivanovii. Other isolates were L. seeligeri (10), L. innocua (4), L. welshimeri (2) and L. grayi (1). All the isolates were further subjected to a PCR assay for detection of the hlyA gene, the hlyA gene was detected in 7 isolates. Multiplex-PCR serotyping assay revealed three of the strains belonging to serovar group 4b, 4d, 4e, and four to serovar group 1/2a, 3a. The prevalence of L. monocytogenes in raw meat, particularly serogroup 4b, 4d, 4e in present study indicates a potential threat to public health.
dc.subject	Microbiology	en_US
dc.title	Isolation and characterization of Listeria species from raw and processed meats	en_US
dc.type	Journal article	en_US