Prophylactic effect of dietary supplementation of fish oil extracted from Sardinella longiceps on renal dysfunction in alloxan induced diabetic mice (Mus musculus)

Abstract

Diabetes mellitus is a multifactorial metabolic disorder caused due to deficiency of pancreatic hormone insulin, which results in failure to metabolize sugar or due to adequacy of another pancreatic hormone glucagon which results in the increased liver glucose output. Oxidative stress plays exquisite role in diabetes, which results in pathogenesis of diabetes related long term vascular complications which are the main cause of morbidity and mortality in diabetic patients. These vascular complications also result in Diabetic kidney disease (DKD) which is the single most common cause of end stage renal disease (ESRD). The present study was being aimed to evaluate the effect of fish oil on diabetic kidney damage. Diabetes was induced by repetitive intra-peritoneal injection of alloxan (100mg/kg BW). The diabetic mice were fed the commercial pellet diet supplemented with 10 percent laboratory extracted fish oil of Sardinella longiceps for a period of one month. Concentration of various parameters like creatinine, uric acid, urea, protein, albumin and globulin in serum and kidney were estimated. The antioxidant parameters like vitamin C, vitamin E, GSH, SOD and Catalase, lipid peroxidation parameters like TBARS and GGT were also monitored, along with the activity of ALP and ACP. The Altered level of tissue biochemical composition and antioxidant status due to diabetes induced damages was nearly brought down to normal levels with the supplementation of 10 percent Sardinella fish oil.