| dc.description.abstract |
Arsenic resistance in bacteria is basically due to a detoxification system encoded by the ars operon. It has been reported in various grams negative as well as gram positive genera. The E. coli chromosomal ars operon and E. coli plasmid R773 borne ars operons were the first ones to be discovered. The ars operon Staphylococcus aureus plasmid pI 258 which shows only 30 percent structural homology with the E.coli counterpart is representative of gram positive ars operons and is known to show functional homology with the former. Subsequently, the ars operon genes were found in diverse taxa. E.g. Bacillus, Pseudomonas, Acidiphilium multivorum, Corynebacterium, Streptomyces, Thiobacillus, Exiguobacterium, Clostidium, Synechocystis and many others. But this inherent property of arsenic resistance and the presence of ars operon have not yet been reported among the members of the genus Vibrio. We, in our studies came across four Vibrios isolated from marine habitats which showed high levels of resistance towards inorganic arsenic species viz. arsenate (greater than 200mM) and arsenite(3mM). All these strains were PCR positive for arsC gene encoding arsenate reductase using gene specific primers. The size of the amplicon ranged from 353-356 bp and revealed 98-99 percent homology with the chromosomal ars operon E.coli strain W 3110. |
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