Systems metabolic engineering approach for recombinant protein production in microbial cell factories

Abstract

The production of recombinant proteins using genetically engineered microbes are well known. However, integrating systems biology approach such as network-based modeling have enabled to identify all possible pathways that can be rationally engineered to improve protein production and also to reduce the by-product accumulation. Furthermore, by utilizing the insilico systems biology tools, the pathway editing can be easily carried out. Recently the exploration of genome engineering using CRISPR Cas9 technology has enhanced the foreign gene integration as well gene deletion in the genome of several cell factories. Hence, with the systems biology tool and synthetic biology approach superior organism can be created, which has ability to produce the recombinant protein in the range of grams per liter. In the present book chapter, we have discussed the constraint-based methods, which can be used for strain improvement. Further, we have briefly described the in vivo gene manipulation techniques used for bacteria as well as yeast system.