A deep-sea hydrogen peroxide-stable alkaline serine protease from Aspergillus flavus

Damare, S.; Mishra, A.; D'Souza-Ticlo-Diniz, D.; Krishnaswamy, Akhila; Raghukumar, C.

dc.contributor.author	Damare, S.
dc.contributor.author	Mishra, A.
dc.contributor.author	D'Souza-Ticlo-Diniz, D.
dc.contributor.author	Krishnaswamy, Akhila
dc.contributor.author	Raghukumar, C.
dc.date.accessioned	2020-11-17T05:58:25Z
dc.date.available	2020-11-17T05:58:25Z
dc.date.issued	2020
dc.identifier.citation	3 Biotech. 10(12); 2020; ArticleID_528.	en_US
dc.identifier.uri	https://doi.org/10.1007/s13205-020-02520-x
dc.identifier.uri	http://irgu.unigoa.ac.in/drs/handle/unigoa/6280
dc.description.abstract	We report here the production of an alkaline serine protease by Aspergillus flavus isolated at 5600-m depth from deep-sea sediments of the Central Indian Basin. When grown on defatted groundnut oil meal at 30 degrees C for 48-72 h, this fungal isolate produced 2000-2500 ACU mL sup(-1) of alkaline protease. The purified protease had activity optima at pH 10.0 and 45 degrees C. It was a thiol-independent serine protease, identified as an alkaline serine protease ALP1 with a molecular mass of 42.57 kDa. The thermostability and activity of the enzyme increased at 60 degrees C, in the presence of additives such as sucrose, Tween 20, sorbitol, Ca sup(2+) and glycerol and was not adversely affected by H sub(2) O sub(2) indicating its potential as a detergent additive.	en_US
dc.publisher	Springer	en_US
dc.subject	Biotechnology	en_US
dc.title	A deep-sea hydrogen peroxide-stable alkaline serine protease from Aspergillus flavus	en_US
dc.type	Journal article	en_US
dc.identifier.impf	y