Aminoglycoside antibiotic kanamycin functionalized tetraphenylethylene molecular probe for highly selective detection of bovine serum albumin protein

Abstract

A novel tetraphenylethylene (TPE) functionalized aminoglycoside antibiotic kanamycin (TPE-kana 1) has been successfully synthesized and characterized by means of modern analytical and spectroscopic techniques. The probe TPE-kana 1 showed strong affinity towards bovine serum albumin (BSA) compared to its other biological competitors. The recognition of BSA have been investigated employing UV-Vis absorption and fluorescence emission spectroscopy. The significant color change of TPE-kana 1 with BSA can be observed by necked eye, where the role of AIE-active TPE molecule is handle in both optical and colorimetric changes. The quenching of fluorescence of TPE-kana 1 with BSA was characterized by fluorescence spectroscopy, with 71.16 percent of quenching efficiency. Moreover, the Stern-Volmer quenching constant was calculated and found to be 2.46x10 sup(7) M sup(-1). Probe TPE-kana 1 showed detection limit of 2.87 nM (nM) towards BSA with binding constant 7.56x10 sup(7) M. A molecular docking study is also performed to investigate the detail interactions between TPE-kana 1 with the sites of BSA via non-covalent i.e., H-bonding, Pi-cation interactions, Pi-donor hydrogen bonds and Pi-Pi interactions. The lowest binding energy conformation was found at - 10.42 kcal/mol.