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Isolation of a thermophilic Meiothermus sp. strain JE2 and its infective phage vB_Msp_JE2 from Rajwadi hot spring, India

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dc.contributor.author Faria, N.
dc.contributor.author Ghadi, S.C.
dc.date.accessioned 2023-07-19T06:59:09Z
dc.date.available 2023-07-19T06:59:09Z
dc.date.issued 2021
dc.identifier.citation Indian Journal of Biotechnology. 20; 2021; 355-363. en_US
dc.identifier.uri http://nopr.niscpr.res.in/handle/123456789/61880
dc.identifier.uri http://irgu.unigoa.ac.in/drs/handle/unigoa/7052
dc.description.abstract A thermophilic, red-pigmented aerobic bacteria designated as strain JE2 was isolated from the water sample of the Rajwadi hot spring. Bacterial strain JE2 was Gram-negative, rod-shaped that grew optimally at pH 7.5 and 60 degrees C. Molecular identification of the 16S rRNA sequence indicated that bacterial strain JE2 formed a coherent cluster with genus Meiothermus. Subsequently, a lytic bacteriophage designated as vB_Msp_JE2 was isolated from the Rajwadi hot spring that could be propagated using Meiothermus sp. strain JE2. One-step growth curve using Meiothermus sp. strain JE2 as host demonstrated a latent period of 70 min and a burst size of 20. Bacteriophage vB_Msp_JE2 was stable at pH 6.0 - 8.0. Additionally, the phage was thermostable at 60 degrees C and was completely inactivated above 75 degrees C. Since, most microbial studies have been reported predominantly from the Himalayan geothermal province, the present study reports the association of a unique culturable pigmented thermophilic bacterial host and bacteriophage from Rajwadi hot spring present in the West coast geothermal province, India. en_US
dc.publisher NIScPR-CSIR, India en_US
dc.subject Biotechnology en_US
dc.title Isolation of a thermophilic Meiothermus sp. strain JE2 and its infective phage vB_Msp_JE2 from Rajwadi hot spring, India en_US
dc.type Journal article en_US
dc.identifier.impf y


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