Alpha-santonin 1,2-reductase and its role in the formation of dihydrosantonin and lumisantonin by Pseudomonas cichorii S

Naik, U.P.; Mavinkurve, S.

dc.contributor.author	Naik, U.P.
dc.contributor.author	Mavinkurve, S.
dc.date.accessioned	2015-06-02T09:58:06Z
dc.date.available	2015-06-02T09:58:06Z
dc.date.issued	1987
dc.identifier.citation	Canadian Journal of Microbiology. 33(8); 1987; 658-662.	en_US
dc.identifier.uri	http://dx.doi.org/10.1139/m87-115
dc.identifier.uri	http://irgu.unigoa.ac.in/drs/handle/unigoa/94
dc.description.abstract	1,2-Dihydrosantonin is the first stable product in the degradative pathway of alpha-santonin byPseudomonas cichorii S. Its formation is catalyzed by an oxidoreductase, which is NADH or NADPH dependent and has an apparent Km value of 66.66 mu M for santonin and 44.33 mu M for NADH. The enzyme activity is stable at pH 6.0, 7.0, and 8.0, and is not affected by EDTA and divalent metal ions. It is postulated that the enzymic reduction of santonin occurs via formation of a transient zwitterionic intermediate, which undergoes nonenzymatic 1,4-sigmatropic rearrangement to yield lumisantonin during the solvent extraction process. Lumisantonin is, thus, not a true metabolic intermediate but an artifact.	en_US
dc.publisher	NRC Research Press (Canadian Science Publishing)	en_US
dc.subject	Microbiology	en_US
dc.title	Alpha-santonin 1,2-reductase and its role in the formation of dihydrosantonin and lumisantonin by Pseudomonas cichorii S	en_US
dc.type	Journal article	en_US
dc.identifier.impf	y