Abstract:
A bacterial isolate capable of utilizing tributyltin chloride (TBTC) as sole source of carbon was isolated from marine water samples. The isolate, producing a soluble green pigment, was identified as Pseudomonas aeruginosa strain USS25 NCIM-5224. The isolate showed maximum growth with 2 mm of TBTC in mineral salt medium. Time course results showed complete elimination of TBTC after 75 days of incubation.During the growth on TBTC, a product (280 mg) was found to accumulate, which was extracted with chloroform and detected on thin-layer chromatography. Based on the IR, NMR spectra and GC-MS analysis, the isolated product was identified as monobutyltin dichloro hydride.
