Abstract:
In the present study we report the use of cells of a Haloarchaeon for the green synthesis of silver nanoparticles. Biosynthesis of AgNPs occurred within 30 s on exposure of cells of Haloferax alexandrinus to silver nitrate in direct sunlight. Maximum AgNPs production was achieved within 4 min of exposure of silver nitrate (0.05 percent) to cells (5 mg/ml), at pH 7, at ambient day temperature (26-34 degrees C). The AgNPs had characteristic surface plasmon resonance at 420 nm in UV-Vis spectra. Spherical and irregular crystals ranging from 2 to 60 nm in size with an average size of 18 nm were observed in TEM analysis. The FTIR spectral analysis indicated involvement of N-H, -OH, C=O, C-O functional groups present in cells of Haloferax alexandrinus MTCC 3265. The biogenic AgNPs exhibited broad spectrum antimicrobial activity against human and mammalian pathogens, in the order of Pseudomonas aeruginosa ATCC 9027 greater than Bordetella bronchiseptica ATCC 4617 greater than Bacillus subtilis ATCC 6633 greater than Staphylococcus aureus ATCC 6538P greater than Staphylococcus epidermidis ATCC 12228 greater than Escherichia coli ATCC 8739 greater than Salmonella typhimurium ATCC 14028.
