Identification of genes specifying proline biosynthesis In marine bacterium Alteromonas haloplanktis

Abstract

A gene bank of total DNA of a marine bacterium, Alteromonas haloplanktis, was constructed on pBR322. Two hybrid plasmids pUS2010 and pUS2011 carrying inserts of 8.2 and 5.7 kb, respectively, were isolated that complemented the proBA deletion in E. coli CSH26. Restriction map of the inserts showed that both plasmids in common carried a 5.7 kb fragment. This restriction fragment thus contains both the genes involved in proline biosynthesis in A. haloplanktis and could be expressed in E. coli.