Abstract:
Diabetes mellitus is a multifactorial disorder and prolonged suffering from the diabetes leads to damage the several vital organs due to excessive oxidative stress. On the other hand, omega-3 polyunsaturated fatty acids prevent the oxidative stress as evidenced from the various literatures. Therefore, we hypothesized the dietary intake of omega-3 rich fish oil could be beneficial in the management of diabetes and thus protect the several organs from the oxidative damages. Diabetes was induced in Swiss albino mice, Mus musculus, by repeating the intra peritoneal injection of Alloxan (100mg / kg body weight). Half of the diabetic mice were fed ad libitum the pellet feed blended with 10 percent laboratory extracted fish oil from Sardinella longiceps for one month in the laboratory. Various biochemical parameters pertaining to oxidative stress were measured along with the histology of the various organs like heart, kidney, liver and pancreas and the expression of certain cytokines and other m RNAs was measured by one step reversed PCR. The results suggest the prophylactic effect of dietary intake of Sardinella oil in diabetic mice by regulating the expression of cytokines and other marinas along with the increase of ant oxidant molecules in various organs.
