Abstract:
Sargassum tenerrimum was collected from the shores of Vainguinim in Goa. Preliminary phytochemical investigations of this seaweed revealed the presence of alkaloids which was further confirmed by their chromatographic analysis. Alkaloids were extracted in methanol and re-precipitated in Cone. Ammonium hydroxide. The precipitate was dissolved in Chloroform and it was observed that alkaloids were retained and resolved as a single band even after treatment with charcoal for removal of pigments. Sequential fractionation (water, chloroform) of the chloroform extract resulted in identification of four alkaloids Ephedrine, Cuscohygrine, Pyrvinium and Doxapram by LC-MS.
