Screening antioxidant activity by in-situ HPTLC-DPPH assay and in-vitro cytotoxic assessment of Annona muricata L. plant organ extracts on MCF-7 and SCC-40 cell lines

Abstract

Annona muricata Linn exhibits immense array of medicinal and ethno-pharmaceutical benefits owing to the synthesis of secondary metabolites like alkaloids, phenols and acetogenins with prospective biological activity. In this work, we analyzed the potential antioxidant and cytotoxic efficacy through synergistic interaction possessed by phytoconstituents in crude methanol extracts of A. muricata plant organs. The rind, pulp, seed, leaf, bark and root extracts of A. muricata were evaluated by rapid free-radical scavenging activity of developed bioprofile using HPTLC method with post-chromatographic derivatization with DPPH reagent to assess the active antioxidant profile in plant parts. While, the in-vitro cytotoxicities of extract were studied employing MTT assay on MCF-7 and SCC-40 cancer cell lines; HPTLC-DPPH assay showed presence of distinct yellowish bands against violet chromatographic background confirming in-situ anti-oxidant activity in all plant organs. In-vitro anti-cancer assay demonstrated strong cytotoxic potential in all plant organs studied. The leaf extract showed better cytoxicity toward MCF-7 cells with IC sub(50) value of 13.04 Mu g/mL while seed extract exhibited better efficacy with IC sub(50) of 15.07 Mu g/mL against SCC-40 cell lines. The findings indicate that phytochemicals present in A. muricata organs might be responsible for bioactivity and indeed be used as a potential source of effective natural anti-oxidant and anti-cancer agent in nutrition and pharmaceutical industries.