Abstract:
This study evaluates the biosynthesis of gold nanoparticle (GNP) using Antarctic bacteria and assesses its potential antibacterial activity on sulfate-reducing bacteria (SRB). The GNPs were biosynthesized at distinct temperatures (4 degrees, 10 degrees, 25 degrees, 30 degrees and 37 degrees C) using bacterial isolate GL1.3, obtained from Antarctic lake water. Biochemical and phylogenetic analysis concluded that the isolate GL1.3 belongs to Bacillus sp. The GNP biosynthesis was achieved at all the incubation temperatures (4 degrees, 10 degrees, 25 degrees, 30 degrees and 37 degrees C) only during the log phase of growth. These formed nanoparticles were identified by UV-Visible spectroscopy, scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), transmission electron microscopy (TEM) and X-ray diffraction (XRD) to be of size 30-50 nm. These GNPs exhibited antibacterial activity against SRB (Desulfovibrio sp.) evaluated by broth micro-dilution method. At 200 Mu g mL sup(-1) GNP concentrations, being the minimal inhibitory concentration (MIC), the growth rate and sulfate reducing activity of Desulfovibrio sp. were reduced by 12 percent and 7 percent respectively. Comet assay revealed that the genotoxic effect of GNP on SRB is responsible for the inhibition of its growth and sulfide production. This showed that the Antarctic microbes could be useful for GNP synthesis even under psychrophilic conditions for various biomedical applications.
