Abstract:
Bacterial strain VB3, an aerobic bacterium, was isolated from the water of Vashisht hot spring, Himachal Pradesh, India. The lysogenic bacterial strain VB3's draft genome sequences comprise approximately 6.06 MB DNA and 5607 predicted genes with a G+C content of 60.56 percent. The 16S rRNA sequencing assigned the bacterial strain VB3 to the genus Pseudomonas. Pseudomonas sp. VB3 was a Gram-negative, rod-shaped bacteria demonstrating catalase, oxidase, citrate utilization, cellulase, and lipase activities. Genome mining revealed fourteen gene clusters coding for the biosynthesis of bioactive secondary metabolites. PHASTER analysis identified two intact and three incomplete putative prophage-related sequence regions in the draft genome. The reduction in the growth rate of the strain VB3 on mitomycin-C treatment was concurrent with the detection of the clearance zone in the spot assay.
