Release of periplasmic proteins from Sphingomonas paucimobilis strain S ATCC 43388

Abstract

Alkaline phosphotase and oc-santonin binding proteins were released in the periplasmic shock fluid from S. paucimobilis strain S ATCC 43388, on treatment with freeze thaw (FTM), cold osmotic shocking (COSM) and chloroform (CHLM) method. Such cells devoid of periplasmic proteins showed corresponding reduction in the transport of a-santonin. Amongst the methods employed FTM was found to be most effective.