Abstract:
A high frequency plant regeneration system was developed for the production of high yielding elite clones of Garcinia indica via direct organogenesis. A maximum number of 86.2 shoot buds per explant were induced from the mature seed segments cultured on Woody plant medium (WPM) supplemented with 2.0 mg dm(-3) N(6)-benzyladenine and 1 mg dm(-3) indole-3-acetic acid. Rooting was achieved on half-strength WPM medium supplemented with 3 mg dm(-3) indole-3-butyric acid. Rooted plantlets were acclimatized and transferred to greenhouse for further growth. The highest survival rate of 95 percent was recorded using a mixture of garden soil and sand. Histological studies clearly revealed multiple shoot formation from mature seed segments.
